Analytics & QC
Material without data is just a tube of liquid.
Every production run ships with the basic characterisation that tells you what is actually in the vial. Affinity and specificity work is available on top — for our material or yours.
5working days
Standalone analytics turnaround
BLI
Label-free kinetics: kon, koff, KD
WB
Western blot validation
Included
Basic QC on every production run
Included as standard
What comes with every run, at no extra cost.
We do not sell basic QC back to you as an upgrade. If we cannot show you what we made, we have not finished the job.
| SDS-PAGE | Reduced and, for antibodies, non-reduced. Supplied as an image, not a claim. |
|---|---|
| Concentration | A280 with the extinction coefficient calculated from your sequence. |
| Size-exclusion profile | Aggregation state and monomer content for polished material. |
| Endotoxin | Bacterial batches only, where it is a real risk. Measured and reported; low endotoxin is our standard on the E. coli route, not an upgrade. |
| Batch record | Host, strain or cell line, construct, buffer, volume and date. |
Add-on 01
BLI affinity measurement
Bio-layer interferometry: label-free, real-time binding kinetics. One partner is immobilised on a biosensor, the other flows past, and the instrument watches the interaction happen.
Typical uses
- Rank a panel of designed binders before committing to the best one
- Confirm that a computational affinity prediction survives contact with reality
- Compare a new batch against the previous one
- Check that a tag or a mutation did not cost you binding
from €1,200
BLI affinity, one interaction pair
- Association and dissociation rates
- Affinity constant with the fit shown
- Raw sensorgrams supplied
- Reference subtraction and controls
Add-on 02
Western blot validation
Does your antibody find the target in a denatured sample, and does it find anything else? A specificity check against the lysate or recombinant standard you specify.
A Western blot answers a narrow question. It tells you about recognition of denatured epitopes. It will not tell you about binding to the native, folded protein — for that you want BLI.
from €480
Western blot, one target
- Sample and control lanes
- Blot image supplied
- Molecular weight markers
- Conditions documented for repeatability
Questions
About affinity and validation work
What exactly does BLI give me?
Association rate, dissociation rate and the affinity constant derived from them, plus the raw sensorgrams and the fit. You get the data, not just a number in an email — including the traces that did not fit well, if any.
What do you need from me to run BLI?
Both binding partners, or one of them plus an order for us to make the other. Tell us which one you want immobilised and whether either carries a tag we can use as a capture handle. If you are not sure, send both and we will propose a setup.
Can I order analytics without ordering production?
Yes. Send us purified material and we will run it — roughly 5 working days. This is common when a group has protein from an earlier campaign and wants an independent measurement.
Is BLI the same as SPR?
They answer the same question by different means, and for most ranking and screening work they agree. BLI needs less sample handling and copes better with crude-ish samples; SPR is often preferred for publication-grade kinetics on tight interactions. We run BLI and will tell you if your question genuinely needs SPR.
What can Western blot tell me here?
Whether your antibody recognises the target in a denatured context, and whether it picks up anything else in the lysate you care about. It is a specificity check, not a quantitative assay.
Is endotoxin measured?
On bacterial batches, yes, as standard — endotoxin comes from the E. coli cell wall, and low endotoxin is how we work on that route rather than something you order separately. The measured value is reported with the batch. Mammalian runs have no bacterial host, so endotoxin is not part of their standard QC panel; if you need the measurement on CHO or HEK material anyway, ask at the quote stage.
Already have the protein?
Send it over and we will characterise it. Independent measurement on material you made yourself is a perfectly normal order here.